Table 1 Nucleotide sequences of real-time PCR primers and TaqMan probes
mRNA | Accession No | Amplicon size | Forward primer (5' to 3') | TaqMan probe (TET – BHQ1) | Reverse primer (5' to 3') |
|---|---|---|---|---|---|
Ttp/Tis11/Zfp36 | NM_011756 | 70 bp | GGTACCCCAGGCTGGCTTT | AACTCAATATAATCCTGCCTTAGCCTT | ACCTGTAACCCCAGAACTTGGA |
Zfp36l1/Tis11b | NM_007564 | 60 bp | TGCGAACGCCCACGAT | ACCACCACCCTCGTGTCCGCC | CTTCGCTCAAGTCAAAAATGG |
Zfp36l2/Tis11d | NM_001001806 | 77 bp | GAGGGCACCTCCCAACCT | TTGCAATTTCGACCATTACAGGACCCA | TGACAGAAGTGTGGTCGACATTT |
Zfp36l3 | NM_001009549 | 70 bp | CGAACTGCGTACCCTGTCAAG | CACCCCAAGTACAAGACGGAGCCTTG | GCCAACGCTGTGGAAGGT |
Tnf | NM_013693 | 66 bp | GCTGTCGCTACATCACTGAACCT | TGCTCCCCACGGGAGCCG | TGACCCGTAGGGCAATTACA |
Ptgs2/Cox2 | NM_011198 | 106 bp | CCACCTCTGCGATGCTCTTC | AGCTGTGCTGCTCTGCGCT | CATTCCCCACGGTTTTGACATG |
Csf2/Gm-csf | NM_009969 | 71 bp | CACCCGCTCGCCCAACCC | TCACCCGGCCTTGGAAGCATGTAGA | GGAGGCTCAGAGCTTCTTTGA |
Elavl1/Hua/Hur | NM_010485 | 69 bp | CCTCCGAGCCCATCACAGT | AAGTTTGCAGCCAATCCCAACCAGAA | GCGAGAGGAGAGCCATGTTT |
Vegfa | NM_001025250 | 68 bp | CAAAAACGAAAGCGCAAGAAA | CCCGGTTTAAATCCTGGAGCG | CGCTCTGAACAAGGCTCACA |
Vegfb | NM_011697 | 83 bp | GATCCAGTACCCGAGCAGTCA | TGTCCCTGGAAGAACACAGCCAATGTG | TCTCCTTTTTTTTTGGTCTGCAT |
Rpl32 | NM_172086 | 66 bp | AACCGAAAAGCCATTGTAGAAA | AGCAGCACAGCTGGCCATCAGAGTC | CCTGGCGTTGGGATTGG |