Figure 1

Representative histology of LPS-induced lung injury in CD-1 mice. H&E staining of left lung lobe from a female mouse following a single i.p. injection of 0.2 ml of 0.25 mg/ml LPS for 2 hours (B) or 24 hours (D) or PBS (vehicle) for 2 hours (A) or 24 hours (C). No histopathologic findings were observed in the lungs of 2-hour PBS-treated control mice (A). One 24-hour PBS (vehicle)-treated control mouse (C) had a focal incidental mononuclear cell infiltrate present perivascularly within the lung. Perivascular edema and the accumulation of mixed cell infiltrates within blood and lymphatic vessels, as well as in the adjacent interstitium at both 2-hours (B) and 24-hours (D) following LPS treatment. A minimal increase in the number of alveolar macrophages (asterisk) was also observed in the 24-hour LPS-treated mice.