Skip to main content
Figure 1 | Journal of Inflammation

Figure 1

From: C-reactive protein does not opsonize early apoptotic human neutrophils, but binds only membrane-permeable late apoptotic cells and has no effect on their phagocytosis by macrophages

Figure 1

Purity and functional integrity of FITC-CRP. (a) Native and FITC-conjugated CRP migrated as single bands of approximately 23 kD in SDS-PAGE. (b) Western blot of CRP and known amounts of human IgG on a 9% acrylamide gel under non-reducing conditions. The blot was probed with anti-human IgG-HRP. CRP contained <0.1% IgG (w/w). MW markers in kDa are illustrated. (c) FITC-CRP bound to phosphorylcholine-coated beads in the presence of 2 mM Ca2+ (dark shaded histogram). Binding in 5 mM EDTA (light shaded histogram) and BSA-coated beads (control; open histogram) is also shown. (d) FITC-CRP bound to freeze-thawed necrotic neutrophils (shaded histogram). Binding of FITC-BSA is shown as a control (open histogram).

Back to article page

Journal of Inflammation

ISSN: 1476-9255

Contact us