Figure 1

IL-9 RT-PCR. A. Time course. PBMC were incubated for various times with OKT3, RNA was extracted, IL-9 real time RT-PCR was performed, and the mean threshold cycle (Ct) was determined. The data shown are from an experiment on one representative individual. The values are means of duplicate determinations. B. Gel electrophoresis. PBMC were incubated for 24 h with or without OKT3 and with or without Dex (10-6 M). RNA was extracted and IL-9 RT-PCR performed for 40 cycles. For each condition, duplicate PCRs were performed on cDNA from one representative individual. Products were analysed in a 2% agarose gel. The left lane contains HaeIII cut ΦX174 molecular size markers (Roche); the arrow indicates the position of the 281/271 bp markers.