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Figure 7 | Journal of Inflammation

Figure 7

From: Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response

Figure 7

Expression of proteins involved in TLR signaling in stimulated primary small-airway-epithelial cells (SAEC). (A) SAEC were untreated (control) or stimulated for 24 h with the indicated TLR ligands. MyD88, TOLLIP, TRIF, TIRAP, IRAK-1 and IRAK-2 protein expression was analyzed by Western blotting. A vimentin loading control is shown below. (B) SAEC were stimulated for 1 h, 3 h or 6 h with poly(I:C) (I:C) and TRIF protein levels were analyzed by Western blotting (upper panel). Results were compared to untreated controls (U) and to IRAK-1 protein expression (lower panel).

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