Figure 2

Effect of dominant negative Nurr1 on Nurr1 induced inflammatory gene expression. Demonstration that Nurr1 causes increased expression of IL-8, AREG and KITLG mRNA that can be attenuated with the coexpression of Nurr1-D/N in K4IM cells using Taqman Quantitative RT-PCR. Values expressed represent the mean fold change ± SEM, compared to the blank vector control and is derived from three experiments normalised in each case to GAPDH gene expression at 24 hr post transfection. Similar results were observed in a further two independent experiments.