Figure 4

RV-D1’s effects on Poly I:C-induced IκBα mRNA expression levels in HCE cells. HCE cells were stimulated in SFM with Poly I:C (25 μg/cc) for 12 hours after cells pre-incubated for 2 hours with RV-D1’s 1 μM. mRNA expression levels of IκBα were examined by real time PCR. The mRNA expression levels are presented in a logarithmic scale, data are expressed as percentage (mean ± SD) of the Poly I:C stimulus which was normalized to 1.0 from three independent experiments (n = 3). Asterisks indicate a significant difference (P < 0.05) between groups compared to poly I:C only.