Figure 3

RV-D1’s effects on poly I:C-induced cytokines mRNA expression levels in HCE cells, examined by real-time PCR (A–D). mRNA expression levels are presented on a logarithmic scale. For TNF-α and IL-1β detection the HCE cells were incubated in SFM with RV-D1 for 12 hours and cells pre-incubated for 2 hours with RV-D1 at 1 μM (A, C). For IL-6 and IL-8 detection the HCE cells were incubated in SFM with Poly I:C for 3 hours and cells pre-incubated for 2 hours with RV-D1’s at 1 μM (B, D). Data is expressed as percentage (mean ± SD) of the expression found after poly I:C stimulation, which was normalized to 1.0, and derived from four independent experiments (n = 4). Asterisks indicate a significant difference (P < 0.05) between groups compared to poly I:C only.