Figure 6

HCE and HCF cells following activation with poly I:C and LPS respectively. A. HCE cells response to poly I:C stimulation. HCE cells were incubated with poly I:C. The protein content of pro-inflammatory cytokines was measured using cytometric bead array technique (A). The cytokine-specific mRNA content was measured using real-time PCR (B). The data shown are representative of triplicate experiments. All values are expressed as mean ± SD. The asterisk (p < 0.05), double asterisk (p < 0.01) and three asterisks (p < 0.001) represent statistical significance for experiments as compare to cells incubated in medium alone. B. HCF cells response to LPS stimulation. The protein content of pro-inflammatory cytokines was measured using cytometric bead array technique (Figure 7A). Cytokine-specific mRNA content was measured using real-time PCR. The data shown are representative of triplicate experiments (Figure 7B). All values are expressed as mean ± SD. The asterisk (p < 0.05) and the double asterisk (p < 0.01) represent statistical significance for experiments as compare to medium.