Role of toll-like receptors in human iris pigment epithelial cells and their response to pathogen-associated molecular patterns

Table 3 TLR specific primers and RT-PCR conditions

Genes	Sequence (5′ to 3′ direction)	Annealing temperature (°C)	Cycles	Amplicon size (bp)
TLR1	F: AAAAGAAGACCCTGAGGGCC	62	35	340
	R: TCTGAAGTCCAGCTGACCCT	62		340
TLR2	F: GTACCTGTGGGGCTCATTGT	62	35	191
	R: CTGCCCTTGCAGATACCATT	62		191
TLR3	F: AAATTGGGCAAGAACTCACAGG	60	35	320
	R: GTGTTTCCAGAGCCGTGCTAA	60		320
TLR4	F: TACAAAATCCCCGACAACCTC	60	35	264
	R: AGCCACCAGCTTCTGTAAACT	60		264
TLR5	F: TGCATTAAGGGGACTAAGCCTC	60	35	351
	R: AAAAGGGAGAACTTTAGGGACT	60		351
TLR6	F: TCTTGGGATTGAGTGCTATGA	60	35	337
	R: GTCGTTTCTATGTGGTTGAGG
TLR7	F: TCCAGTGTCTAAAGAACCTGG	60	38	352
	R: TGGTAAATATACCACACATCCC
TLR8	F: TAATAGGCTGAAGCACATCCC	60	35	621
	R: TCCCAGTAAAACAAATGGTGAG
TLR9	F: GTGCCCCACTTCTCCATG	60	35	260
	R: GGCACAGTCATGATGTTGTTG
TLR10	F: TGACCACAATTCATTTGACTACTC	60	35	478
	R:TTGAATACTTTTGGGCAAGCACC
GAPDH	F:ACCACAGTCCATGCCATCAC	60	28	452
	R:TCCACCACCCTGTTGCTGTA
Genes	Sequence	Accession number	Cycles	Amplicon size (bp)
TLR2*	Sequences refer to Qiagen	NNM_003264	45	92
TLR3*		NNM_003265	45	90
TLR4*		NNM_138554	45	111
TLR6*		NNM_006068	45	78
GAPDH*		NNM_001256799	45	95

F = forward primer; R = reverse primer; bp = base pair. *Primer mix used for real-time PCR.

ISSN: 1476-9255