Table 1 Antibodies used for Western blotting

TLR1

Goat IgG

biotin

-

R&D systems

0.2

TLR2

Goat IgG

biotin

-

R&D systems

0.2

TLR3

Mouse IgG_1, k

-

40C1285.6

Imgenex

2

TLR4

Goat IgG

biotin

-

R&D systems

0.2

TLR5

Mouse IgG_2a, k

-

19D759.2

Imgenex

2

TLR6

Rabbit IgG

-

-

Imgenex

2

TLR7

Rabbit IgG

-

-

Imgenex

2

TLR8

Mouse IgG_1, k

-

44C143

Imgenex

2

TLR9

Mouse IgG_1, k

-

26C593.2

Imgenex

2

TLR10

Mouse IgG₁

-

158C1114

Imgenex

2

Isotype

Mouse IgG_2a, k

-

-

eBioscience

10

GAPDH

Mouse IgG₁

-

GAPDH 1D4

Imgenex

1:1000 dilution