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Table 1 Antibodies used for Western blotting

From: Role of toll-like receptors in human iris pigment epithelial cells and their response to pathogen-associated molecular patterns

Antigen specificity Antibody subtype Label Clone Manufacturer Final concentration (μg/ml) or dilution
TLR1 Goat IgG biotin - R&D systems 0.2
TLR2 Goat IgG biotin - R&D systems 0.2
TLR3 Mouse IgG1, k - 40C1285.6 Imgenex 2
TLR4 Goat IgG biotin - R&D systems 0.2
TLR5 Mouse IgG2a, k - 19D759.2 Imgenex 2
TLR6 Rabbit IgG - - Imgenex 2
TLR7 Rabbit IgG - - Imgenex 2
TLR8 Mouse IgG1, k - 44C143 Imgenex 2
TLR9 Mouse IgG1, k - 26C593.2 Imgenex 2
TLR10 Mouse IgG1 - 158C1114 Imgenex 2
Isotype Mouse IgG2a, k - - eBioscience 10
GAPDH Mouse IgG1 - GAPDH 1D4 Imgenex 1:1000 dilution