Expression of TLR2, −3, −4 and −6 mRNA and proteins in human IPE and RPE. Human IPE and RPE (A) from the same donor were cultured to confluence and expression of TLR2, −3, −4 and −6 mRNA and proteins was investigated by real-time PCR (A, B and C) and Western blotting (D, E and F) using specific primers and antibodies, respectively. The concentration of genes was measured using the comparative threshold cycle number and normalised to that of the GAPDH with ARPE-19 (B) and THP-1 (C) served as positive controls. Data represents mean ± SD (N = 3). Two-way ANOVA and Bonferroni’s multiple comparison test was used to analyse the data for A, **p < 0.01, ****p < 0.0001. One-way ANOVA and Turkey’s multiple comparison test was used to analyse the data for B and C, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Results are representative of three independent experiments (the results are consistent from three donors).