Nitrite assay. HEKa cell culture supernatants were collected and treated with Griess Reagent and absorbance was measured at 570nm. Measurements were taken at 4 and 8 hours. Measurements are reported in μM. ANOVA and Tukey’s Honestly Significant Difference were performed to identify statistically significant differences. Results represent the mean and standard deviation of three independent experiments. (#) indicates a statistically significant difference between the PCM and BCM and (*) indicates a statistically significant difference between media and BCM (p < 0.05).