Figure 7

Effect of HA on HAC phosphoproteins. A) Time course (minutes) of the appearance of phosphorylated p38 (black) and JNK (white) in HAC after the addition of IL-1β. B) – F) Dose response of the effect on levels of the indicated signalling proteins of the hexadecylamide derivative of HA added one hour before (white markers, solid line) or simultaneously with (black markers, dashed line) of IL-1β (2 ng/mL) to cultures of HAC. Cultures were harvested after 15 minutes with IL-1β corresponding to peak levels (A). P < 0.05 for differences from cultures with IL-1β alone (no added HA; #) or differences +/− pre-incubation (§). Values are mean% change from IL-1 alone in cells from five separate patients.