Figure 7

Dose-dependent effects of lipopolysaccharide (LPS) on the expressions of surfactant protein-A (SP-A) mRNA and protein for 8 h. HK-2 cells were exposed to 0, 0.1, 1, 2, 5, and 10 μg/mL of LPS for 8 h. (A) Total RNA was prepared for RT-PCR analysis of SP-A mRNA and for gel electrophoresis of SP-A1 and SP-A2 mRNA. (B) Comparison of SP-A1 and SP-A2 mRNA after exposure to different concentrations of LPS for 8 h. (C) Comparison of the SP-A protein after exposure to different concentrations of LPS for 8 h. Each value represents the mean ± SEM for n = 3. The symbol “a” indicates that the value significantly (P < 0.05) differed from the control group. M, 100 bp DNA marker.