Design of the “Small Intestinal Segment Perfusion” (SISP) experiment. (A) Surgically applied jejunal loops were perfused without (control) or with Salmonella (infected) and segments were dissected after the indicated hours (0, 2, 4, or 8) . For all three pigs’ (2-4) treatments of intestinal loops were identical. (B) Isogenic microarray comparisons between segments dissected from infected and control loops after 8 h of perfusion. (C) Interanimal microarray comparisons of Salmonella perfused loops. Segments were dissected after 2 and 4 hours, and at both these time-points 3 interanimal microarray comparisons were performed.