Figure 6

Influence of 1F7 mAb treatment on monocyte endotoxin tolerance. Monocytes from 10 healthy donors were pre-treated with 100 ng/ml LPS or 1.92 μg/ml 1F7 mAb for 18 h, washed with LPS-free PBS and incubated for an additional 4 h with 1 μg/ml LPS or 1.92 μg/ml 1F7 mAb. Levels of TNF-α (a) and IL-10 (b) in supernatants were then measured. All data shown represent mean values ± SE. Significant differences by repeated ANOVA measures are noted between NT, LPS or 1F7 treatment (*p < 0.05) and between LPS re-stimulated pairs (#p < 0.05).