Figure 2

Effect of 1F7 mAb on PBMC apoptosis and necrosis. PBMC were incubated with 1.92 μg/ml 1F7 mAb or IgMκ mAb control for 72 h and analyzed by flow cytometry for Annexin V⁺, propidium iodide (PI)^- apoptotic cells (a) or PI⁺ necrotic cells (b). Data shown in (a) represent mean values ± SE, n = 10. The shaded area of a representative flow cytometry plot (b) corresponds to IgMκ control mAb-treated cells (13.9% PI⁺) and the non-shaded area corresponds to 1F7 mAb-treated cells (12.0% PI⁺).