Figure 1

Time and dose-dependent effects of 1F7 mAb on IL-10 production by PBMC in vitro. PBMC from 10 individuals were incubated with 0.48-1.92 μg/ml 1F7 mAb or IgMκ control mAb for 18 h (a) or with 1.92 μg/ml mAb 1F7 or control for 24, 48 and 72 h (b). The IL-10 concentration in cell free supernatants was then measured by ELISA. Data shown represent mean values ± SE. Mean IL-10 levels significantly different between 1F7 mAb-treated and IgMκ mAb control-treated cell supernatants are noted (*p < 0.05).