Effect of XH-14 on LPS-induced MAPK and AP-1 activation. RAW 264.7 cells were plated in 100 mm-diameter dishes. After 12 h of seeding, cells were treated with different doses of XH-14 for 1 h, followed by stimulation with 100 ng/ml of LPS for 30 min. (A) Whole cell extracts, (B) cytosolic proteins and (C) nuclear proteins were immunoblotted with the indicated Abs. β-actin and poly ADP ribose polymerase were used as a control. The bands were quantified using NIH image analysis software and their relative intensity was expressed as fold-change against the image of the LPS-stimulated RAW 264.7 cells.