Effects of XH-14 on LPS-induced NO and PGE
release. RAW 264.7 cells were treated with 0–20 μM of XH-14 in the presence of 100 ng/ml of LPS or with LPS alone for 24 h, and (A) NO and (B) PGE2 release was determined. The results are reported as mean ± SEM of three independent experiments in triplicate. Statistical significance is based on the difference when compared with LPS-stimulated cells (*P < 0.05, **P < 0.01, ***P < 0.001). Thirty micrograms of protein obtained from each cell lysate was resolved by 10% SDS-PAGE for (C) iNOS and (D) COX-2 determination. β-actin expression is shown as a loading control. The bands were quantified using NIH image analysis software and their relative intensity was expressed as fold against the image of the LPS-stimulated RAW 264.7 cells.