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Table 1 IL-12p40 siRNA specifically silences mRNA for IL-12p40 and not other cytokines.

From: Efficient delivery of small interfering RNA for inhibition of IL-12p40 expression in vivo

Target Silencing by treatmenta
LPS/IFN-γ: - + +
SiRNA: IL-12p40 Mut.siRNA IL-12p40
β-Actin - - -
IL-12p40 - - +
IL-12p35 - - -
IL-23p19 - - -
IFN-γ - - -
IFN-β - - -
TNF-α - - -
IL-4 - - -
IL-5 - - -
IL-6 - - -
IL-10 - - -
  1. aJ774 cells were incubated with or without LPS/IFN-γ and specific or control siRNA as described in the materials and methods section. mRNA for different targets were detected by sqRT-PCR. In this table silencing (+) is defined as the loss of a visible band from stimulated cultures; the - symbol indicates either no loss of a visible band from stimulated (siRNA + LPS/IFN-γ) cultures, or no visible alteration (induction or loss) in unstimulated (siRNA – LPS/IFN-γ) cultures. All results represent at least two experiments performed in triplicate.