Heat shock protein 70 down-regulates the production of toll-like receptor-induced pro-inflammatory cytokines by a heat shock factor-1/constitutive heat shock element-binding factor-dependent mechanism
- Eduardo Ferat-Osorio1, 2,
- Aldair Sánchez-Anaya1,
- Mireille Gutiérrez-Mendoza1,
- Ilka Boscó-Gárate1,
- Isabel Wong-Baeza1, 3,
- Rodolfo Pastelin-Palacios6,
- Gustavo Pedraza-Alva5,
- Laura C Bonifaz1,
- Pedro Cortés-Reynosa4,
- Eduardo Pérez-Salazar4,
- Lourdes Arriaga-Pizano1,
- Constantino López-Macías1,
- Yvonne Rosenstein5, 7Email author and
- Armando Isibasi1, 8Email author
© Ferat-Osorio et al.; licensee BioMed Central Ltd. 2014
Received: 27 December 2013
Accepted: 5 July 2014
Published: 12 July 2014
Heat shock protein 70 (Hsp70) is an intracellular chaperone protein with regulatory and cytoprotective functions. Hsp70 can also be found in the extracellular milieu, as a result of active secretion or passive release from damaged cells. The role of extracellular Hsp70 is not fully understood. Some studies report that it activates monocytes, macrophages and dendritic cells through innate immune receptors (such as Toll-like receptors, TLRs), while others report that Hsp70 is a negative regulator of the inflammatory response. In order to address this apparent inconsistency, in this study we evaluated the response of human monocytes to a highly purified recombinant Hsp70.
Human peripheral blood monocytes were stimulated with Hsp70, alone or in combination with TLR agonists. Cytokines were quantified in culture supernatants, their mRNAs were measured by RT-PCR, and the binding of transcription factors was evaluated by electrophoretic mobility shift assay (EMSA). Kruskal-Wallis test or one-way or two-way ANOVA were used to analyze the data.
The addition of Hsp70 to TLR-activated monocytes down-regulated TNF-α as well as IL-6 levels. This effect was independent of a physical interaction between Hsp70 and TLR agonists; instead it resulted of changes at the TNF-α gene expression level. The decrease in TNF-α expression correlated with the binding of HSF-1 (heat shock transcription factor 1, a transcription factor activated in response to Hsp70) and CHBF (constitutive HSE-binding factor) to the TNF-α gene promoter.
Extracellular Hsp70 negatively regulates the production of pro-inflammatory cytokines of monocytes exposed to TLR agonists and contributes to dampen the inflammatory response.
KeywordsHeat shock protein 70 Inflammatory response HSF-1 CHBF Toll-like receptors
Inflammation occurs in response to infection, heat shock or cellular stress. During infection, increased levels of Heat shock protein 70 (Hsp70) confer cytoprotection  by inhibiting components of inflammatory signaling pathways, such as the NF-κB transcription factor . Hsp70 is predominantly an intracellular protein, but it can be released to the extracellular milieu as a result of tissue damage or cellular necrosis . Moreover, the presence of extracellular Hsp70 in the absence of cell death, suggests that live cells actively secrete Hsp70 . It has been proposed that Hsp70 functions as an alarmin , as it can be detected in the plasma of healthy individuals and at higher concentrations in the serum of severely traumatized patients, patients with autoimmune diseases and inflammatory conditions, and children with septic shock [6–9]. The transcription of Hsp70 is mediated by the activation of heat shock transcription factor-1 (HSF-1), which binds to the heat shock element (HSE) in the Hsp70 gene promoter . Interestingly, HSF-1 is a negative regulator of TNF-α release in a mouse model of lipopolysaccharide (LPS)-induced shock, and it represses the transcription of mouse TNF-α and IL-1β . The identification of a functional HSE in the human TNF-α promoter suggests that HSF-1 directly participates in the regulation of TNF-α expression [12, 13].
Increased levels of extracellular Hsp70 have been reported to stimulate an inflammatory response via a Toll-like receptor 2 (TLR2)/TLR4/CD14-dependent mechanism that leads to NF-κB activation, and TNF-α, IL1-β and IL-6 production . However, other reports show that extracellular Hsp70 induces LPS tolerance and prevents the augmentation of pro-inflammatory cytokines levels that follows LPS stimulation . The immune-stimulating activity of Hsp70 through TLR2 and TLR4 remains thus controversial, and it has been proposed that the pro-inflammatory activity of Hsp70 results rather of contaminating LPS . Furthermore, a pro-inflammatory role of extracellular Hsp70 would be difficult to reconcile with the role of intracellular Hsp70, which mainly regulates apoptosis and confers cytoprotection [1, 17]. In fact, data from the HSP70 1/3 knockout mice suggests that extracellular Hsp70 is important for the negative regulation of inflammatory mediators during systemic infection .
In this study, we evaluated the role of extracellular Hsp70 on the inflammatory response elicited by different TLR agonists central to the early onset of innate immune responses to bacterial infections.
Recombinant human stress induced Hsp70 (Hsp72) was purchased from Enzo Life Sciences (formerly Assay Designs, and formerly Stressgen Biotechnologies Corporation). We tested conventional, non-LPS-free Hsp70 (NSP-555), which we will refer to as “HE/Hsp70”; and LPS-free Hsp70 (ESP-555), which we will refer to as “Hsp70”. The non-LPS free HE/Hsp70 contains 200 pg of LPS per μg of protein, while the LPS-free Hsp70 contains 1.4 pg of LPS per μg of protein (fewer than 50 IU of LPS), as assessed by the Limulus amebocyte lysate assay in our laboratory. According to the manufacturer, both Hsp70preparations retain their ATPase activity. To heat-denature Hsp70, the protein was boiled for 120 min. Escherichia coli 0111:B4 LPS was from Sigma Chemical Co. (St. Louis, MO, USA); Salmonella typhimurium flagellin and Staphylococcus aureus peptidoglycan were from Invivogen (San Diego, CA, USA), and vaccine-grade Salmonella enterica serovar Typhi porins, with < 200 pg of LPS per μg of protein, were obtained in our laboratory as previously reported .
Cell separation and stimulation
Peripheral blood mononuclear cells from leukocyte concentrates of healthy blood bank donors were isolated with Lymphoprep™ (Axis-Shield, Oslo, Norway). Mononuclear cells were resuspended in 2 ml of RPMI 1640 supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (all reagents were from GIBCO Invitrogen, Carlsbad, CA, USA), counted and plated at 20 × 106 cells per 15 ml in a culture dish for 2 h at 37°C. The monocytes-enriched fraction was detached with cold buffer (NaCl 0.8%, KCl 0.04%, glucose 0.1%, sodium EDTA 0.02% and NaHCO3 0.6%) and plated at 1 × 106 cells per well in 24-well plates for 24 h at 37°C and 5% CO2 in supplemented RPMI. All experiments were performed with mononuclear cells enriched for monocytes by at least 85% as assessed by flow cytometry expression.
After 24 h, the culture medium was removed and cells were washed twice with phosphate-buffered saline before adding fresh supplemented RPMI with or without LPS (100 ng/ml), peptidoglycan (10 μg/ml), flagellin (10 μg/ml) or porins (3 μg/ml). The TLR agonists were added alone or in the presence of the indicated amounts of Hsp70 in 1 ml of RPMI, for the indicated times. Supernatants were then collected, centrifuged (3,000 rpm for 5 min), and frozen at −70°C. Alternatively, TLR agonists were pre-incubated with Hsp70 in 200 μl of binding buffer (25 mM TRIS, 20 mM HEPES, 47.5 mM KCl, and 2.25 mM Mg(OAc)2, pH 7.15) for two hours at 37°C, before adding them to the cells.
Cell separation and stimulation for flow cytometric analysis of TLR expression
Peripheral blood mononuclear cells were obtained from leukocyte concentrates of healthy blood-bank donors by gradient centrifugation with Lymphoprep™ (Axis-Shield, Oslo, Norway). Mononuclear cells were resuspended in 2 ml of RPMI 1640 supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin (all reagents from GIBCO Invitrogen, Carlsbad, CA, USA), counted and seeded at 0.5 × 106 cells per tube and incubated in a humidified incubator with 5% CO2 and 37°C.
The concentrations of TNF-α, IL-6 and IL-10 in cell culture supernatants were measured with ELISA kits (BD Biosciences Pharmingen, San Jose, CA, USA), according to the manufacturers’ protocols.
The TNF-α mRNA was analyzed by RT-PCR. Total RNA was extracted from monocytes using TRIzol reagent (Invitrogen). The concentration and purity of RNA were determined at 260 and 280 nm. Single-stranded cDNA was synthesized by mixing 1 μg RNA with 1 μl of oligo-dT(12–18) (0.5 μg/μl, Invitrogen), 1 μl of a dNTP mixture (10 mM each), 2 μl of 0.1 M dithiotreitol and 200 U of SuperScript II RNase H reverse transcriptase in a total volume of 20 μl; the reaction mixture was incubated at 42°C for 50 min, followed by 70°C for 15 min to inactivate the reverse transcriptase. cDNA (1 μg) was used as a template for PCR amplification of the TNF-α gene. The PCR reaction mixture consisted of 0.5 μl of a dNTP mixture (10 mM each), 1.5 μl of 25 mM MgCl2, 1 μl of primer working stock (10 pmol of each primer), 0.25 μl of dimethilsulfoxide (Sigma) and 1.25 U of Taq DNA polymerase in a total volume of 20 μl (all reagents were from Promega, Madison, WI, USA, unless otherwise indicated). The TNF-α primers were 5′-GGT-GCT-TGT-TCC-TCA-GCC-TC-3′ and 5′-CAG-GCA-GAA-GAG-CGT-GGT-G-3′  and the β-actin primers were 5′-GTG-GGG-CGC-CCC-AGG-CAC-CA-3′ and 5′-CTC-CTT-AAT-GTC-ACG-CAC-GAT-TTC-3′ . β-actin was used as an internal control in each experiment. The reaction mixture was denatured at 95°C for 10 min, followed by 35 cycles of denaturation (95°C/30 s), annealing (58°C/1 min), and extension (72°C/1 min); the final extension was for 10 min. The PCR products were separated by electrophoresis in agarose gels at 70 V for 60 min, stained with ethidium bromide and observed by ultraviolet illumination. Images were captured and analyzed with an IS-1000 Digital Imaging System (Alpha-Innotech Corporation, San Leandro, CA, USA). The pixel densities of TNF-α and β-actin bands were determined and the ratios of TNF-α to β-actin were calculated; these ratios represent the relative expression levels or expression index of TNF-α mRNA.
Electrophoretic mobility shift assay (EMSA)
To prepare nuclear extracts, monocytes were lysed in 1 ml of cold buffer A (10 mM Tris–HCl pH 7.4, 10 mM NaCl, 6 mM MgCl2, 10 mM NaF, 1 mM Na3VO4, 1 mM dithiotreitol, and 1 mM phenylmethanesulfonyl fluoride) and incubated on ice for 10 min. After this incubation, 10 μl of IGEPAL 10% were added, and the tubes were incubated for another 5 min at 4ºC on a shaking platform. The lysates were centrifuged at 2,600 rpm for 5 min, resuspended in 40 μl of cold buffer B (20 mM HEPES, pH 7.9, 420 mM NaCl, 20% glycerol, 1.5 mM MgCl2, 0.2 mM EDTA, 1 mM Na3VO4, 10 mM NaF, 1 mM DTT, and 0.2 mM PMSF) and incubated for 15 min at 4ºC on a shaking platform. Nuclear extracts were recovered by centrifugation at 12,000 rpm for 15 min at 4°C and frozen at −70°C. Total protein concentration was measured using a commercial reagent (Bio-Rad Laboratories, Hercules, CA, USA) and a bovine serum albumin standard curve. To evaluate the binding of HSF-1 to the promoter region of the human TNF-α gene, we used a double-stranded oligonucleotide that corresponds to the +45/+73 position of the TNF-α sequence (5′-AGA-GAA-G CA-ACT-ACA-GAC-CCC-CCC-TGA-AA-3′) and contains specific binding sites for HSF-1 (in boldface). The oligonucleotide was labeled with [γ-32P] ATP with a T4 polynucleotide kinase (Fermentas, Thermo Scientific, Glen Burnie, MD, USA), according to the manufacturer’s protocol. The 32P-labelled oligonucleotide probe (1 ng) was incubated with 5 μg of nuclear extract in the presence of 3 μg poly(dI-dC) in 0.25 M HEPES, pH 7.5, 0.6 M KCl, 50 mM MgCl2, 1 mM EDTA, 7.5 mM DTT and 9% glycerol) for 20 min at 4°C. We added a 50-fold excess of unlabeled TNF-α probe (“cold”) or an irrelevant oligonucleotide (5′-ACG-TGT-GAT-GAA-ATG-CTA-GGC-GAT-C-3′) as specific and non-specific competitors, respectively. After incubation, samples were resolved on 6% polyacrylamide gels in 0.5x Tris borate-EDTA buffer (74.5 mM Tris–HCl, 1.6 mM sodium EDTA, and 44.5 mM boric acid pH 8.5) for 4 h at 0.28 mA. The gels were dried and analyzed by autoradiography with a Kodak intensifying screen (Carestream Health, Rochester, NY, USA) at −80°C.
Fluorescence-activated cell sorting (FACS) analysis of TLR and CD14 expression
FACS was performed to detect cell surface expression of TLR2, TLR4 on CD14 cells after Hsp70 stimulation. Human mononuclear cells were exposed to 3 μg/ml Hsp70 for 1 or 4 hours, and then the cells were harvested and washed twice with PBS containing 1% BSA. Cells were incubated with FITC-conjugated anti-TLR2, PE-conjugated anti-TLR4 (eBioscienced) and Pacific Blue-conjugated anti-CD14 (BioLegend), at room temperature for 30 min. Fluorescence was determined by a FACScan flow cytometry (Becton-Dickinson).
Each experiment was performed three or four times with independent donors. Data were analyzed with GraphPad Prism 5.0 software (GraphPad Software, San Diego, CA, USA). Kruskal-Wallis test, one-way or two-way ANOVA were used as required, followed by Dunn’s multiple comparison test, Tukey or Bonferroni post-tests. A P value < 0.05 was considered statistically significant.
Hsp70 decreases the production of TNF-α by LPS-activated monocytes
Hsp70 has been reported to inhibit some components of the pro-inflammatory signaling cascades . To investigate this possibility, we added LPS-free Hsp70 and LPS to the same well, and found that Hsp70 considerably decreased the amount of TNF-α secreted by monocytes in response to LPS. This effect was lost if the Hsp70 was heat-denatured before the assay (Figure 1C). To assess if Hsp70 prevented LPS-induced TNF-α transcription, we examined the levels of TNF-α mRNA in LPS-activated monocytes. Hsp70 reduced the TNF-α mRNA levels resulting of a 2 or 6 hr LPS stimulation (Figure 1E) with similar results at 1, and 4 h after LPS activation (data not shown). The reduced mRNA levels correlated with lower levels of TNF-α in the supernatants (Figure 1F). Hsp70 had no effect on the surface expression of TLR2 and TLR4 (Additional file 2: Figure S2).
Altogether this data support the hypothesis that LPS-free Hsp70 is not a pro-inflammatory signal for the cell, and furthermore that it dampens TNF-α production.
Hsp70 regulates LPS-dependent TNF-α production through a mechanism that does not depend on the direct interaction between LPS and Hsp70
These data suggests that, depending on the concentration, Hsp70 can override LPS-mediated signals, and that this effect does not depend on the ability of Hsp70 to directly interact with LPS.
Hsp70 decreases the production of TNF-α, but not that of IL-10, in response to TLR agonists
Hsp70-induced binding of HSF-1 to the TNF-α promoter correlates with decreased TNF-α protein levels
An increasing number of diseases result of a dysregulated inflammatory response, and several inflammatory mediators, including Hsp70, have been implicated. The molecular chaperone Hsp70 has many functions depending on the cell type, and our understanding of the roles it may play, especially the extracellular form, remains incomplete, and is even controversial [27–32]. Two members of the HSP70 family, Hsp73 and Hsp72, share a high degree of sequence homology but differ in their expression pattern: Hsp73 is constitutively expressed, while Hsp72 is stress-inducible . In resting cells, Hsp73 is found mainly in the cytoplasm, the nuclei and nucleoli, while Hsp72 is found in nuclei and nucleoli. However, after a stressful event, such as heat shock, the distribution of both forms is homogenized throughout the cell, suggesting that both isoforms associate with similar molecules to protect essential cell structures . Incubation of human monocytes with Hsp70 –the inducible form- was reported to have a functional consequence by eliciting the rapid expression of pro-inflammatory cytokines ; whereas Hsp73 does not induce any significant release of IL-6 – a pro-inflammatory cytokine by monocytes .
Some authors have reported that extracellular Hsp70 functions as a DAMP and activates monocytes, macrophages and dendritic cells, potentially through cell surface receptors such as CD14, CD40, CD91, Lox1, TLR2 and TLR4 . In different experimental models [14, 22, 37–39], Hsp70 has been found to induce intracellular calcium fluxes and NF-κB activation, with the subsequent production of the pro-inflammatory cytokines TNF-α, IL-1β, IL-6 and IL-8. However, Hsp70 is present in the peripheral circulation of healthy subjects at concentrations that, according to these studies, would elicit cytokine production , suggesting that Hsp70 is not pro-inflammatory in all contexts [40, 41]. In fact, intracellular Hsp70 has a cytoprotective effect, as it inhibits the production of inflammatory mediators during cellular stress . Our results support a cytoprotective role for Hsp70 whereby it diminishes the production of the pro-inflammatory mediator TNF-α, through the recruitment of HSF-1 transcription factor to the cytokine promoter, thus extending its cytoprotective role beyond the intracellular space to the extracellular milieu.
Data shown here bring also evidence that extracellular Hsp70 not only fails to induce TNF-α production in human monocytes, but that it down-regulates their capacity to produce TNF-α and IL-6 in response to LPS. The Hsp70 (LPS-free HSP70) we used in our experiments corresponds to the inducible isoform (Hsp72); as a result of a multi-step chromatography purification process it has very low levels of LPS (<50 endotoxin units/mg) and could not, by itself, induce cytokine production by human monocytes. These results are consistent with reports that attribute the immune-stimulating activity of Hsp70 to contaminating LPS [16, 43], and others that show that in patients with rheumatoid arthritis, extracellular Hsp70 decreased IL-6, IL-8 and MCP-1 production induced by TNF-α . Likewise, recombinant Hsp72 (induced Hsp70) has been reported to lessen the severity of collagen-induced arthritis in mice, by lowering serum TNF-α and IL-6 concentration .
We used a highly purified commercial Hsp70 for our experiments (purified by multi-step chromatography, with <50 endotoxin units/mg); this Hsp70 corresponds to the inducible isoform (Hsp72), and it is not bound to any other molecule. However, we cannot exclude the possibility that Hsp70 bound another protein or peptide in the cell culture supernatants. This binding, as well as its ADP/ATP-binding status, could modify the effects of Hsp70 on human monocytes . These two aspects need to be evaluated in future studies. The recombinant Hsp70 that we used is produced in E. coli, so it is possible that its conformation is different from that of native Hsp70 in cells. However, this Hsp70 from Enzo Life Sciences, formerly Stressgen retains its ATPase activity, and we observed that it activates NF-κB through TLR2 and TLR4 signalling, as has been reported previously which suggest that the recombinant protein retains a conformation compatible with its biological activity [16, 47].
Hsp70 interacts with highly hydrophobic peptides . TLR agonists usually have hydrophobic regions that bind to the leucine-rich-repeats (LRR) domain-groove of TLRs . The binding of a TLR agonist to Hsp70 could interfere with its ability to engage its TLR, and the subsequent signaling cascade leading to cytokine production would be defective. Our results do not support this possibility. Data we report here suggest rather that the effect of Hsp70 on LPS-induced TNF-α production is independent of a physical association with LPS, because the addition of LPS after monocytes were treated with Hsp70 did not lead to TNF-α production, even though Hsp70 was no longer present in the system. Furthermore, our results indicating that Hsp70 activates NF-κB through TLR2 and TLR4 signaling argues against the possibility that Hsp70 inhibit LPS-mediate TNF-α expression by negatively modulating TLR4 expression. In fact, we found that Hsp70 has no effect on the surface expression of TLR2 and TLR4 on monocytes, consistent with a report showing that Hsp70 does not affect TLR2 nor TLR4 expression on mouse macrophages .
TNF-α production by human peripheral blood mononuclear cells was reduced by more than 50% when the cells were cultured with LPS in the presence of Hsp70, but that incubating these cells with 15 to 60 μg/ml of Hsp70 for 24 h increased TNF-α production in response to 1 ng/ml of LPS . This apparent discrepancy with our results could be caused by the different concentrations of Hsp70 and LPS used, and by the use of peripheral blood mononuclear cells instead of enriched monocytes for the experiments.
Endotoxemia stimulates stress responses through HSF-1, as a protective mechanism for the host. HSF-1 is found in the cytoplasm as a monomer devoid of transcriptional activity, but upon exposure to a heat shock or to other stressing insults, it is phosphorylated, trimerized and transported to the nucleus, where it binds to HSEs. Extracellular Hsp70 phosphorylates Akt through the TLR4 signaling pathway, resulting in glycogen synthase kinase (GSK)-3β inactivation . The inactivation of GSK-3β, an inhibitory regulator of HSF-1, leads to increased HSF-1 activity . CHBF interacts also with HSE and competes with HSF1 for binding to DNA . The mouse TNF-α promoter contains an HSE binding site for HSF-1 and binding of HSF-1 this HSE results in repressed transcription . Based on the location of the putative HSE in the human TNF-α gene promoter, HSF-1 and CHBF could hinder the RNA polymerase processivity . In our experimental system, we provide evidence that the decrease in TNF-α production caused by Hsp70 in LPS-activated monocytes results of a change at the gene expression level: the amount of TNF-α mRNA was decreased in the presence of Hsp70. We report that monocyte co-stimulation with LPS and Hsp70 lead to HSF-1 and CHBF binding to the HSE on the TNF-α promoter, and that this binding correlated with a decreased production of TNF-α. As expected, unstressed cells contained mainly the slower migrating complex. These data are consistent with the fact that heat shock, which increases the levels of HSF-1, has a suppressive effect on the inflammatory response, possibly because HSF-1 exerts a competitive inhibition on NF-κB binding . We observed that Hsp70 activates NF-κB through TLR2 and TLR4 signaling (Additional file 1: Figure S1). However, the activation of this transcription factor did not lead to TNF-α production, probably as a result of the strong activation of HSF-1. The reduction of IL-6 production in our model could be explained because HSF-1 inhibits the expression of this cytokine through activating transcription factor 3 (ATF-3) .
Interestingly, we found that Hsp70 treatment prevented LPS-induced IL-6 expression but not that resulting from engaging TL2 (porins) o TLR5 (flagellin). Unlike TLR2 and TLR5, TLR4-induced IL-6 expression involves the activation of the JAK-STAT pathway, thus making IL-6 expression susceptible to the negative regulation of SOCS1 . Noteworthy, T. gondii Hsp70 promotes SOCS1 expression in macrophages . Thus, it is possible that by inducing SOCS1 expression H70 specifically preventsTRL-4 induced JAK-STAT-dependent IL-6 expression, without affecting that resulting from TLR2 or TLR5 signaling.
A previous paper reported that exogenous recombinant Hsp70 interacts with HSF-1 in cell lines. The authors find that Hsp70 binds to HSF-1 and blocks its DNA-binding activity, and they suggest a model in which this association prevents the transcription of HSF-1-regulated genes in resting cells. After a heat shock, miss-folded proteins compete for Hsp70 binding, and this releases HSF-1 . In our study, we did not explore the signalling pathways that lead to HSF-1 activation, but a similar mechanism could operate during inflammation-induced cell stress. Other studies report that HSF-1 deficient mice have increased levels of TNF-α after stimulation with LPS , and that Escherichia coli-induced inflammation is resolved faster when a heat shock precedes inoculation with bacteria . HSF-1 also inhibits the transcription and secretion of G-CSF in response to LPS . Furthermore, HSF-1 has been reported to prevent the overproduction of pro-inflammatory cytokines in conditions such as sepsis , potentially decreasing the inflammation-associated damage. Our results support the idea that exogenous HSP70 provides a mechanism for controlling the excessive expansion of an inflammatory response after monocytes activation by bacterial pathogens through the recruitment of HSF-1 to the human TNF-α promoter, and underscores a potential role for exogenous Hsp70 as a prophylactic agent for different types of inflammatory diseases associated with infections.
Extracellular Hsp70 negatively regulates the production of pro-inflammatory cytokines of monocytes exposed to TLR agonists and contributes to dampen the inflammatory response.
Heat shock protein 70
Reverse transcription polymerase chain reaction
Electrophoretic mobility shift assay
Tumor necrosis factor alfa
Nuclear factor kappa B
Heat shock transcription factor 1
Heat shock element
Constitutive HSE-binding factor.
This work was funded by grants from the Consejo Nacional de Ciencia y Tecnologia (CONACyT; CB-2009-01-134501), the Instituto de Ciencia y Tenologia del Disitrito Federal (ICyTDF; PICDSI09-270) and the Instituto Mexicano del Seguro Social (lMSS) Health Research Fund Mexico (FIS; FIS/IMSS/PROT/G10/859 and FIS/IMSS/PROT/G11/954) awarded to CLM. The authors thank Luz María Rocha-Ramírez, PhD, for valuable suggestions.
- Bruemmer-Smith S, Stuber F, Schroeder S: Protective functions of intracellular heat-shock protein (HSP) 70-expression in patients with severe sepsis. Intensive Care Med. 2001, 27 (12): 1835-1841.PubMedView ArticleGoogle Scholar
- Chen H, Wu Y, Zhang Y, Jin L, Luo L, Xue B, Lu C, Zhang X, Yin Z: Hsp70 inhibits lipopolysaccharide-induced NF-kappaB activation by interacting with TRAF6 and inhibiting its ubiquitination. FEBS Lett. 2006, 580 (13): 3145-3152.PubMedView ArticleGoogle Scholar
- Basu S, Binder RJ, Suto R, Anderson KM, Srivastava PK: Necrotic but not apoptotic cell death releases heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NF-kappa B pathway. Int Immunol. 2000, 12 (11): 1539-1546.PubMedView ArticleGoogle Scholar
- Lancaster GI, Febbraio MA: Exosome-dependent trafficking of HSP70: a novel secretory pathway for cellular stress proteins. J Biol Chem. 2005, 280 (24): 23349-23355.PubMedView ArticleGoogle Scholar
- Campisi J, Leem TH, Fleshner M: Stress-induced extracellular Hsp72 is a functionally significant danger signal to the immune system. Cell Stress Chaperones. 2003, 8 (3): 272-286.PubMedPubMed CentralView ArticleGoogle Scholar
- Johnson JD, Fleshner M: Releasing signals, secretory pathways, and immune function of endogenous extracellular heat shock protein 72. J Leukoc Biol. 2006, 79 (3): 425-434.PubMedView ArticleGoogle Scholar
- Njemini R, Lambert M, Demanet C, Mets T: Elevated serum heat-shock protein 70 levels in patients with acute infection: use of an optimized enzyme-linked immunosorbent assay. Scand J Immunol. 2003, 58 (6): 664-669.PubMedView ArticleGoogle Scholar
- Pockley AG, Shepherd J, Corton JM: Detection of heat shock protein 70 (Hsp70) and anti-Hsp70 antibodies in the serum of normal individuals. Immunol Invest. 1998, 27 (6): 367-377.PubMedView ArticleGoogle Scholar
- Wheeler DS, Fisher LE, Catravas JD, Jacobs BR, Carcillo JA, Wong HR: Extracellular hsp70 levels in children with septic shock. Pediatr Crit Care Med. 2005, 6 (3): 308-311.PubMedView ArticleGoogle Scholar
- Kiang JG, Tsokos GC: Heat shock protein 70 kDa: molecular biology, biochemistry, and physiology. Pharmacol Ther. 1998, 80 (2): 183-201.PubMedView ArticleGoogle Scholar
- Cahill CM, Waterman WR, Xie Y, Auron PE, Calderwood SK: Transcriptional repression of the prointerleukin 1beta gene by heat shock factor 1. The Journal of biological chemistry. 1996, 271 (40): 24874-24879.PubMedGoogle Scholar
- Singh IS, He JR, Calderwood S, Hasday JD: A high affinity HSF-1 binding site in the 5′-untranslated region of the murine tumor necrosis factor-alpha gene is a transcriptional repressor. J Biol Chem. 2002, 277 (7): 4981-4988.PubMedView ArticleGoogle Scholar
- Xie Y, Chen C, Stevenson MA, Auron PE, Calderwood SK: Heat shock factor 1 represses transcription of the IL-1beta gene through physical interaction with the nuclear factor of interleukin 6. J Biol Chem. 2002, 277 (14): 11802-11810.PubMedView ArticleGoogle Scholar
- Asea A, Kraeft SK, Kurt-Jones EA, Stevenson MA, Chen LB, Finberg RW, Koo GC, Calderwood SK: HSP70 stimulates cytokine production through a CD14-dependant pathway, demonstrating its dual role as a chaperone and cytokine. Nat Med. 2000, 6 (4): 435-442.PubMedView ArticleGoogle Scholar
- Aneja R, Odoms K, Dunsmore K, Shanley TP, Wong HR: Extracellular heat shock protein-70 induces endotoxin tolerance in THP-1 cells. J Immunol. 2006, 177 (10): 7184-7192.PubMedView ArticleGoogle Scholar
- Gao B, Tsan MF: Endotoxin contamination in recombinant human heat shock protein 70 (Hsp70) preparation is responsible for the induction of tumor necrosis factor alpha release by murine macrophages. J Biol Chem. 2003, 278 (1): 174-179.PubMedView ArticleGoogle Scholar
- Beere HM, Green DR: Stress management - heat shock protein-70 and the regulation of apoptosis. Trends Cell Biol. 2001, 11 (1): 6-10.PubMedView ArticleGoogle Scholar
- Singleton KD, Wischmeyer PE: Effects of HSP70.1/3 gene knockout on acute respiratory distress syndrome and the inflammatory response following sepsis. Am J Physiol Lung Cell Mol Physiol. 2006, 290 (5): L956-L961.PubMedView ArticleGoogle Scholar
- Salazar-Gonzalez RM, Maldonado-Bernal C, Ramirez-Cruz NE, Rios-Sarabia N, Beltran-Nava J, Castanon-Gonzalez J, Castillo-Torres N, Palma-Aguirre JA, Carrera-Camargo M, Lopez-Macias C, Isibasi A: Induction of cellular immune response and anti-Salmonella enterica serovar typhi bactericidal antibodies in healthy volunteers by immunization with a vaccine candidate against typhoid fever. Immunol Lett. 2004, 93 (2–3): 115-122.PubMedView ArticleGoogle Scholar
- Hayashi F, Means TK, Luster AD: Toll-like receptors stimulate human neutrophil function. Blood. 2003, 102 (7): 2660-2669.PubMedView ArticleGoogle Scholar
- Butch AW, Chung GH, Hoffmann JW, Nahm MH: Cytokine expression by germinal center cells. J Immunol. 1993, 150 (1): 39-47.PubMedGoogle Scholar
- Asea A, Rehli M, Kabingu E, Boch JA, Bare O, Auron PE, Stevenson MA, Calderwood SK: Novel signal transduction pathway utilized by extracellular HSP70: role of toll-like receptor (TLR) 2 and TLR4. The Journal of biological chemistry. 2002, 277 (17): 15028-15034.PubMedView ArticleGoogle Scholar
- Quintana FJ, Cohen IR: Heat shock proteins as endogenous adjuvants in sterile and septic inflammation. J Immunol. 2005, 175 (5): 2777-2782.PubMedView ArticleGoogle Scholar
- Cervantes A, Galvan IM, Isasi P: AMPSO: a new particle swarm method for nearest neighborhood classification. IEEE Trans Syst Man Cybern B Cybern. 2009, 39 (5): 1082-1091.PubMedView ArticleGoogle Scholar
- Kim D, Ouyang H, Yang SH, Nussenzweig A, Burgman P, Li GC: A constitutive heat shock element-binding factor is immunologically identical to the Ku autoantigen. J Biol Chem. 1995, 270 (25): 15277-15284.PubMedView ArticleGoogle Scholar
- Li GC, Yang SH, Kim D, Nussenzweig A, Ouyang H, Wei J, Burgman P, Li L: Suppression of heat-induced hsp70 expression by the 70-kDa subunit of the human Ku autoantigen. Proc Natl Acad Sci U S A. 1995, 92 (10): 4512-4516.PubMedPubMed CentralView ArticleGoogle Scholar
- Ganter MT, Ware LB, Howard M, Roux J, Gartland B, Matthay MA, Fleshner M, Pittet JF: Extracellular heat shock protein 72 is a marker of the stress protein response in acute lung injury. Am J Physiol Lung Cell Mol Physiol. 2006, 291 (3): L354-L361.PubMedPubMed CentralView ArticleGoogle Scholar
- Johnson JD, Campisi J, Sharkey CM, Kennedy SL, Nickerson M, Fleshner M: Adrenergic receptors mediate stress-induced elevations in extracellular Hsp72. J Appl Physiol. 2005, 99 (5): 1789-1795.PubMedView ArticleGoogle Scholar
- Kim HP, Morse D, Choi AM: Heat-shock proteins: new keys to the development of cytoprotective therapies. Expert Opin Ther Targets. 2006, 10 (5): 759-769.PubMedView ArticleGoogle Scholar
- Lee KJ, Kim YM, Kim DY, Jeoung D, Han K, Lee ST, Lee YS, Park KH, Park JH, Kim DJ, Hahn JH: Release of heat shock protein 70 (Hsp70) and the effects of extracellular Hsp70 on matric metalloproteinase-9 expression in human monocytic U937 cells. Exp Mol Med. 2006, 38 (4): 364-374.PubMedView ArticleGoogle Scholar
- Mortaz E, Redegeld FA, Nijkamp FP, Wong HR, Engels F: Acetylsalicylic acid-induced release of HSP70 from mast cells results in cell activation through TLR pathway. Exp Hematol. 2006, 34 (1): 8-18.PubMedView ArticleGoogle Scholar
- Schmitt E, Gehrmann M, Brunet M, Multhoff G, Garrido C: Intracellular and extracellular functions of heat shock proteins: repercussions in cancer therapy. J Leukoc Biol. 2007, 81 (1): 15-27.PubMedView ArticleGoogle Scholar
- Arispe N, Doh M, De Maio A: Lipid interaction differentiates the constitutive and stress-induced heat shock proteins Hsc70 and Hsp70. Cell Stress Chaperones. 2002, 7 (4): 330-338.PubMedPubMed CentralView ArticleGoogle Scholar
- Ellis S, Killender M, Anderson RL: Heat-induced alterations in the localization of HSP72 and HSP73 as measured by indirect immunohistochemistry and immunogold electron microscopy. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2000, 48 (3): 321-332.View ArticleGoogle Scholar
- Bethke K, Staib F, Distler M, Schmitt U, Jonuleit H, Enk AH, Galle PR, Heike M: Different efficiency of heat shock proteins (HSP) to activate human monocytes and dendritic cells: superiority of HSP60. J Immunol. 2002, 169 (11): 6141-6148.PubMedView ArticleGoogle Scholar
- Binder RJ, Vatner R, Srivastava P: The heat-shock protein receptors: some answers and more questions. Tissue Antigens. 2004, 64 (4): 442-451.PubMedView ArticleGoogle Scholar
- Basu S, Binder RJ, Ramalingam T, Srivastava PK: CD91 is a common receptor for heat shock proteins gp96, hsp90, hsp70, and calreticulin. Immunity. 2001, 14 (3): 303-313.PubMedView ArticleGoogle Scholar
- Becker T, Hartl FU, Wieland F: CD40, an extracellular receptor for binding and uptake of Hsp70-peptide complexes. J Cell Biol. 2002, 158 (7): 1277-1285.PubMedPubMed CentralView ArticleGoogle Scholar
- Delneste Y, Magistrelli G, Gauchat J, Haeuw J, Aubry J, Nakamura K, Kawakami-Honda N, Goetsch L, Sawamura T, Bonnefoy J, Jeannin P: Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation. Immunity. 2002, 17 (3): 353-362.PubMedView ArticleGoogle Scholar
- Tanaka S, Kimura Y, Mitani A, Yamamoto G, Nishimura H, Spallek R, Singh M, Noguchi T, Yoshikai Y: Activation of T cells recognizing an epitope of heat-shock protein 70 can protect against rat adjuvant arthritis. J Immunol. 1999, 163 (10): 5560-5565.PubMedGoogle Scholar
- van Eden W, van der Zee R, Prakken B: Heat-shock proteins induce T-cell regulation of chronic inflammation. Nat Rev Immunol. 2005, 5 (4): 318-330.PubMedView ArticleGoogle Scholar
- Barquera Cervera Simón C-NI, Rivera Juan RRy: Obesidad en México: epidemiología y políticas de salud para su control y prevención. Gac Med Mex. 2010, 146: 397-407.PubMedGoogle Scholar
- Bangen JM, Schade FU, Flohe SB: Diverse regulatory activity of human heat shock proteins 60 and 70 on endotoxin-induced inflammation. Biochem Biophys Res Commun. 2007, 359 (3): 709-715.PubMedView ArticleGoogle Scholar
- Luo X, Zuo X, Zhou Y, Zhang B, Shi Y, Liu M, Wang K, McMillian DR, Xiao X: Extracellular heat shock protein 70 inhibits tumour necrosis factor-alpha induced proinflammatory mediator production in fibroblast-like synoviocytes. Arthritis Res Ther. 2008, 10 (2): R41-PubMedPubMed CentralView ArticleGoogle Scholar
- Luo X, Zuo X, Mo X, Zhou Y, Xiao X: Treatment with recombinant Hsp72 suppresses collagen-induced arthritis in mice. Inflammation. 2011, 34 (5): 432-439.PubMedView ArticleGoogle Scholar
- Bergmeier LA, Babaahmady K, Pido-Lopez J, Heesom KJ, Kelly CG, Lehner T: Cytoskeletal proteins bound to heat-shock protein 70 may elicit resistance to simian immunodeficiency virus infection of CD4(+) T cells. Immunology. 2010, 129 (4): 506-515.PubMedPubMed CentralView ArticleGoogle Scholar
- Vabulas RM, Ahmad-Nejad P, Ghose S, Kirschning CJ, Issels RD, Wagner H: HSP70 as endogenous stimulus of the Toll/interleukin-1 receptor signal pathway. J Biol Chem. 2002, 277 (17): 15107-15112.PubMedView ArticleGoogle Scholar
- Seong SY, Matzinger P: Hydrophobicity: an ancient damage-associated molecular pattern that initiates innate immune responses. Nat Rev Immunol. 2004, 4 (6): 469-478.PubMedView ArticleGoogle Scholar
- Matsushima N, Tanaka T, Enkhbayar P, Mikami T, Taga M, Yamada K, Kuroki Y: Comparative sequence analysis of leucine-rich repeats (LRRs) within vertebrate toll-like receptors. BMC Genomics. 2007, 8: 124-PubMedPubMed CentralView ArticleGoogle Scholar
- Antonova OY, Yurinskaya MM, Funikov SY, Evgen’ev MB, Vinokurov MG: Exogenous heat shock protein HSP70 modulates lipopolysaccharide-induced macrophage activation. Doklady biological sciences: proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. 2013, 452 (1): 320-324.View ArticleGoogle Scholar
- Bjorntorp P: Metabolic implications of body fat distribution. Diabetes Care. 1991, 14 (12): 1132-1143.PubMedView ArticleGoogle Scholar
- Bleharski JR, Kiessler V, Buonsanti C, Sieling PA, Stenger S, Colonna M, Modlin RL: A role for triggering receptor expressed on myeloid cells-1 in host defense during the early-induced and adaptive phases of the immune response. J Immunol. 2003, 170 (7): 3812-3818.PubMedView ArticleGoogle Scholar
- Yang SH, Nussenzweig A, Li L, Kim D, Ouyang H, Burgman P, Li GC: Modulation of thermal induction of hsp70 expression by Ku autoantigen or its individual subunits. Mol Cell Biol. 1996, 16 (7): 3799-3806.PubMedPubMed CentralView ArticleGoogle Scholar
- Song M, Pinsky MR, Kellum JA: Heat shock factor 1 inhibits nuclear factor-kappaB nuclear binding activity during endotoxin tolerance and heat shock. J Crit Care. 2008, 23 (3): 406-415.PubMedView ArticleGoogle Scholar
- Takii R, Inouye S, Fujimoto M, Nakamura T, Shinkawa T, Prakasam R, Tan K, Hayashida N, Ichikawa H, Hai T, Nakai A: Heat shock transcription factor 1 inhibits expression of IL-6 through activating transcription factor 3. J Immunol. 2010, 184 (2): 1041-1048.PubMedView ArticleGoogle Scholar
- Kimura A, Naka T, Muta T, Takeuchi O, Akira S, Kawase I, Kishimoto T: Suppressor of cytokine signaling-1 selectively inhibits LPS-induced IL-6 production by regulating JAK-STAT. Proc Natl Acad Sci U S A. 2005, 102 (47): 17089-17094.PubMedPubMed CentralView ArticleGoogle Scholar
- Mun HS, Aosai F, Norose K, Piao LX, Fang H, Akira S, Yano A: Toll-like receptor 4 mediates tolerance in macrophages stimulated with Toxoplasma gondii-derived heat shock protein 70. Infect Immun. 2005, 73 (8): 4634-4642.PubMedPubMed CentralView ArticleGoogle Scholar
- Abravaya K, Myers MP, Murphy SP, Morimoto RI: The human heat shock protein hsp70 interacts with HSF, the transcription factor that regulates heat shock gene expression. Genes Dev. 1992, 6 (7): 1153-1164.PubMedView ArticleGoogle Scholar
- Xiao X, Zuo X, Davis AA, McMillan DR, Curry BB, Richardson JA, Benjamin IJ: HSF1 is required for extra-embryonic development, postnatal growth and protection during inflammatory responses in mice. EMBO J. 1999, 18 (21): 5943-5952.PubMedPubMed CentralView ArticleGoogle Scholar
- Deak T, Nguyen KT, Fleshner M, Watkins LR, Maier SF: Acute stress may facilitate recovery from a subcutaneous bacterial challenge. Neuroimmunomodulation. 1999, 6 (5): 344-354.PubMedView ArticleGoogle Scholar
- Zhang L, Yang M, Wang Q, Liu M, Liang Q, Zhang H, Xiao X: HSF1 regulates expression of G-CSF through the binding element for NF-IL6/CCAAT enhancer binding protein beta. Mol Cell Biochem. 2011, 352 (1–2): 11-17.PubMedView ArticleGoogle Scholar
- Murapa P, Ward MR, Gandhapudi SK, Woodward JG, D’Orazio SE: Heat shock factor 1 protects mice from rapid death during Listeria monocytogenes infection by regulating expression of tumor necrosis factor alpha during fever. Infect Immun. 2011, 79 (1): 177-184.PubMedPubMed CentralView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.