Fig. 4

Crtc1 overexpression increases LPS-induced inflammation and apoptosis in RAW264.7 cells. (A, B) Western blot (A) and quantification (B) of Crtc1 overexpression in RAW264.7 cells. **P < 0.01; n = 3. (C-F) qRT-PCR showing the impact of Crtc1 overexpression on LPS-induced expression of TNF-α (C), IL-1β (D), IL-6 (E), and CXCL1 (F) in RAW264.7 cells. ^*P < 0.05, ^***P < 0.001 vs. EGFP;^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. Control; n = 6. (G, H) Representative TUNEL staining images (G) and quantification (H) showing the impact of Crtc1 overexpression on LPS-induced apoptosis in RAW264.7 cells. ^***P < 0.001 vs. EGFP; ^###P < 0.001 vs. Control; n = 3. (I-L) Western blot (I) and quantification (J-L) showing the impact of Crtc1 overexpression on LPS-induced alternations in Cleaved-caspase3/Caspase3 (J), Bcl-2 (K) and Bax (L) in RAW264.7 cells. ^*P < 0.05, ^**P < 0.01 vs. EGFP; ^#P < 0.05, ^###P < 0.001 vs. Control; n = 3