Fig. 1

Crtc1 deficiency ameliorated CLP-induced ALI. (A, B) Scatter plot (A) and heat map (B) of Crtc1 mRNA changes in LPS-induced inflammation models from different datasets in the GEO database. (C) Crtc1 knockout was validated through DNA genotyping (upper) and Western blot (lower) in lung tissue. n = 3. (D, E) Representative images (D) and quantitation (E) of Evans blue albumin to evaluate pulmonary vascular permeability in Crtc1 KO mice, compared with WT mice. ^***P < 0.001 vs. Crtc1^+/+; ^###P < 0.001 vs. Sham. n = 6. (F, G) Representative H&E staining images (F) and ALI scoring (G) in lung tissue. ^*P < 0.05 vs. Crtc1^+/+; ^###P < 0.001 vs. Sham. n = 3. (H, I) Cytokine IL-6 (H) and TNF-α (I) in BALF were detected by ELISA. ^***P < 0.001 vs. Crtc1^+/+; ^###P < 0.001 vs. Sham. n = 6. (J-M) Impact of Crtc1 deficiency on CLP-induced Cytokines including TNF-α (J), IL-1β (K), IL-6 (L) and CXCL11 (M) in lung tissue detecting by qRT-PCR. ^*P < 0.05, ^**P < 0.01 vs. Crtc1^+/+; ^##P < 0.01 vs. Sham. n = 6