Fig. 4

ETS exposure contributes to cellular senescence via activation of complement component C3.A Total RNA isolated from the lungs of young C57BL/6J mice (5 months old) was used to screen targets of interest. The heat map presents an overview of dysregulated targets, and the expression of selected genes is reported with bar graphs. B Protein was isolated from lung homogenates and C3 protein expression levels (C3A, C3B, and C3C) were analyzed by Western Blotting. β-ACTIN was used as endogenous control. data are shown as mean ± SEM (n=6/group. *P<0.05, **P<0.01, ***P<0.001 compared with Air+PBS group; ^## P<0.01 compared with Air+BLM group; ⁺ P<0.05, ⁺⁺ P< 0.01, ⁺⁺⁺ P<0.001 compared with ETS+PBS group)