Fig. 3

ETS exposure exacerbates bleomycin-induced overexpression of collagen and LOX. C57BL/6J mice (5 months old) were exposed to ETS for 30 days and then administered with BLM and these parameters were analyzed 14 days-post BLM. Protein expressions were analyzed by Western blotting from lung homogenates or by IHC staining on sequential sections (see methods). A Represented blot images (COL1A1, LOX, and MMP2) are shown. β-ACTIN was used as an endogenous control. Proteins expressions (fold change) are shown as bar graphs. Densitometry analyses are done individually. B Protein (COL1A1, COL4A1, and LOX) abundance and localization were quantified via IHC staining as a positive staining percentage. Data are shown as mean ± SEM (n=5-6/group *P<0.05, **P<0.01, ***P<0.001 compared with Air+PBS group; ^# P<0.05, ^{# #} P<0.01, ^{# # #} P<0.001 compared with Air+BLM group; ⁺ P<0.05, ⁺⁺ P< 0.01, ⁺⁺⁺ P<0.001 compared with ETS+PBS group)