Fig. 1

ETS exposure followed by bleomycin administration showed body weight decline, collagen deposition, and impaired lung function. A C57BL/6J mice (5 and 17 months old) were exposed to ETS for 30 days, and then administered with BLM, and all the parameters were analyzed 14 days-post BLM. B Body weight was monitored during 14 days post-BLM. C Lung mechanics (Resistance, Compliance, and Elastance) were measured during sacrifice on day 14 post-BLM. D Trichrome staining (20x, Scale bar = 100μm) was performed to calculate the collagen deposition (%). Data were presented as mean ± SEM (n=6/young group n=2-3/old group. *P<0.05, **P<0.01, ***P<0.001 compared with Air+PBS group; ^# P<0.05, ^{# #} P<0.01, ^{# # #} P<0.001 compared with Air+BLM group; ⁺ P<0.05, ⁺⁺ P< 0.01, ⁺⁺⁺ P<0.001 compared with ETS+PBS group)