Fig. 5

α7 nAChRs expression in hind paw macrophages (a) Experimental Timeline. Immunohistochemistry of α7^tdT/Iba1⁺ macrophages in CFA-injected and non-injected hind paw skin (subdermal) 8 days post-CFA (day 0) and 7 days post 4R (15 mg/kg) or veh treatment (s.c.) (day 8). (b) A representative image of α7^tdT and Iba1⁺ hind paw subdermal co-expression. Scale bar: 20 µm. c Cell counts normalized to the area of subdermal α7^tdT cells. (d) Cell counts normalized to the area of subdermal Iba1 + cells. (e) Cell counts normalized of colocalized α7^tdT and Iba1⁺. (f) Experimental Timeline. Flow cytometric analysis of hind paw subdermal macrophages (F4/80) and T-cells (CD3) of CFA-injected (ipsi) and non-injected (contra) hind paw skin 8 days post-CFA only. (g) Hind paw skin was enzymatically dissociated and stained with F4/80, CD3, and endogenous α7^tdT. After gating for dissociated hind paw skin cells based on forward and side scatter, cells were differentiated by their tdTomato⁺ signal, then subdivided into F4/80 and CD3 populations. (h) Analysis of overlapping of tdTomato⁺ cells that are either macrophages or T-cells in males and females