Fig. 2
Systemic 4R administration reduces inflammation-induced thermal but not tactile hypersensitivity in female mice (a) Experimental timeline for acetone (cold), Hargreaves (heat) and von Frey (tactile) tests in CFA-injected and uninjected hind paws at days 2 and 7 after 4R (15 mg/kg) or vehicle administration in females. Acetone response score (b, e), withdrawal latency to heat stimulation (c, f) and withdrawal threshold to tactile stimulation (d, g) on experimental days 2 and 7, respectively. All values are expressed as mean ± SEM. n = 6 animals per treatment and test. Two-way ANOVA followed by Å Ãdák’s multiple comparison test; p < 0.01 (**); p < 0.001 (***); p < 0.0001 (****) or Tukey’s multiple comparisons test; adjusted p < 0.0001 (§§§§). Mann–Whitney multiple comparisons test, Holm-Å Ãdák method; adjusted p < 0.05 (#); adjusted p < 0.01 (##). Not significant = ns