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Table 2 Quantification of relative mRNA expression of senescence marker and SASP factors in endothelial cells treated with different conditions for 24 h One-way ANOVA followed by Tukey’s test

From: Colchicine prevents oxidative stress-induced endothelial cell senescence via blocking NF-κB and MAPKs: implications in vascular diseases

Gene

Control

H2O2

Colchicine

H2O2 + Colchicine

P value

P21

1.00 ± 0.06

3.97 ± 0.47

1.93 ± 0.25

3.08 ± 0.34

0.0001

IL-1β

1.10 ± 0.56

2.03 ± 0.10

0.40 ± 0.13

1.15 ± 0.31

0.0024

IL-6

1.00 ± 0.12

2.31 ± 0.28

1.74 ± 0.22

1.67 ± 0.10

0.0003

IL-8

1.01 ± 0.22

1.37 ± 0.22

1.46 ± 0.18

0.73 ± 0.02

0.0039

MCP-1

1.05 ± 0.36

1.87 ± 0.15

1.13 ± 0.19

0.38 ± 0.13

0.0003

ICAM-1

1.02 ± 0.26

2.26 ± 0.31

1.55 ± 0.06

1.54 ± 0.19

0.0012

VCAM-1

1.01 ± 0.18

1.30 ± 0.15

0.72 ± 0.08

1.42 ± 0.11

0.0009

E-Selectin

1.03 ± 0.31

2.07 ± 0.09

0.56 ± 0.06

0.68 ± 0.27

0.0001

MMP-1

1.00 ± 0.04

2.07 ± 0.07

2.81 ± 0.06

3.75 ± 0.06

0.0001

MMP-2

1.00 ± 0.07

1.34 ± 0.07

0.95 ± 0.06

0.82 ± 0.05

0.0001

MMP-8

1.07 ± 0.44

1.66 ± 0.80

1.45 ± 0.30

1.41 ± 0.11

0.5538

MMP-11

1.00 ± 0.13

1.66 ± 0.19

1.05 ± 0.08

1.08 ± 0.18

0.0020

TIMP-1

1.03 ± 0.30

0.90 ± 0.10

1.79 ± 0.33

0.72 ± 0.14

0.0026

TIMP-2

1.00 ± 0.08

1.47 ± 0.24

1.69 ± 0.20

1.58 ± 0.10

0.0056