Fig. 6
Expressions of pro-inflammatory mediators are reduced by cAMP in IgG-IC-treated neutrophils. The mouse receives intraperitoneal injection of 3% of Thioglycollate (BD, USA) dissolved in ddH2O. Four hours later, neutrophils are harvested. Neutrophils are pre-treated with DMSO or Rolipram (10 µM) for 1 h. Then the cells are treated with or without 100 µg/ml of IgG-IC. 3 h later, total cellular RNAs are isolated. Then expressions of TNF-α (A), MIP-1α (B), and MIP-1β (C) are examined. Data are expressed as mean ± SEM (N = 3). * and ** suggest p < 0.05 and 0.01, respectively