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Fig. 5 | Journal of Inflammation

Fig. 5

From: Inhibition of GSK3β activity alleviates acute liver failure via suppressing multiple programmed cell death

Fig. 5

Inhibition of TRAF6 was able to partially reverse the hepatocyte-protective effect of TDZD-8 in vitro. A and (B) Flow cytometry detection of apoptosis levels in each group and its statistical analysis. C and (D) Immunofluorescence detection of RIPK1 and caspase-8 localization and expression levels in vitro and their quantitative analysis (magnification × 400). EG Protein blotting and immunofluorescence to detect MLKL, GSDMD, cleaved caspase-7, cleaved caspase-3 protein levels in each group of cells and their quantitative analysis. *P < 0.05 compare with control group, #P < 0.05 compare with model group, &P < 0.05 compare with TDZD-8 group

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