Fig. 1

Effect of TGF-β1 on ET-1 in A549 cells. Confluent A549 cells were incubated with 10 ng/mL TGF-β1, 100 ng/mL BMP-2, 100 ng/mL BMP-4 and 100 ng/mL BMP-7 respectively. The incubation times were 2 h for real-time qPCR and 12 h for ELISA. The ET-1 mRNA expression is shown in Fig. 1a. The relative ET-1 mRNA levels (normalized to GAPDH mRNA) in TGF-β1, BMP-2 and BMP-7 group were increased than those in control group (*p < 0.05). The results of ELISA assay in Fig. 1b demonstrated that only the TGF-β1 treatment significantly increased the levels of ET-1 protein expression (*p < 0.05). The results are presented as means ± SD of 3 independent experiments performed in triplicate. (C, control group; TGF, TGF-β1 group; BMP-2, BMP-2 group; BMP-4, BMP-4 group; BMP-7, BMP-7 group)