Fig. 2

Detection of two LCN2 glycosylated forms in urine after three days of UUO. Representative images showing the LCN2 protein expression in the right (obstructed) and left (intact) ureters, sera, and urine samples collected from the obstructed side of the renal pelvis or bladder in UUO mice. Each experiment was repeated six times for the sham operation and 10 times for the UUO mice. Ure-R, right ureter; Ure-L, left ureter; Se, serum; p-U, urine in renal pelvis; and b-U, bladder urine. The numbers on the right side of the figures indicate the molecular size of LCN2 (kDa). Renal pelvic urine (p-U) could be obtained only after ureteral obstruction was established. Therefore, the results for pelvic urine are only shown for the UUO group