Figure 2From: Anti-inflammatory effects of methoxyphenolic compounds on human airway cellsEffects of apocynin on cytokine mRNA and protein levels under inflammatory conditions. A, Real time PCR data gathered from mRNA isolated from A549 cells treated with TNF-α (50 ng/ml) in the presence of absence of Apocynin (Apo, 1 mM) for 24 h. Shown are relative levels of inflammatory mediator mRNA in the Apo/TNF-α vs. TNF-α treatment (n = 3; *,p < 0.05; NS, not statistically significant. Cytokine gene expression changes were also measured in BEAS2B cells (B) and PBECs (C), D , Representative raw data from the protein array experiment, comparing cytokine levels in the Apo/TNF-α vs. TNF-α treatment. E, ELISA and densitometeric analysis of cytokine protein array data, comparing cytokine levels in the Apo/TNF-α vs. TNF-α treatment (n = 3; *, p < 0.05; ND, not detected). F, CCL2 expression was measured by ELISA in A549 cells, BEAS2B cells, and PBECs under the treatment conditions described above.Back to article page