| Alveolitisa | PMNb(%+/-SD) | CD4 T cellsb(%+/-SD) | CD8 T cellsb(% +/- SD) | NK cellsb(% +/- SD) |
---|
| WT | Tbet-/- | WT | Tbet-/- | WT | Tbet-/- | WT | Tbet-/- | WT | Tbet-/- |
---|
3 wk SR
| 2.2 × 106+/-1.2 | 1.9 × 106+/-1.9 | 72+/-6.0 | 70+/-14 | 6+/-1.5 | 9+/-2.5 | 3+/-0.7 | 3+/-1.1 | 5+/-1.3 | 2+/-0.1 |
6 wk SR
| 10.3 × 106+/-2.5 | 3.1 × 106 +/-2.2* | 88+/-3.0 | 91+/-1 | 4+/-0.4 | 3+/-1.0 | 2+/-0.3 | 1+/-0.4 | <1 | <1 |
- C57BL/6 or T bet-/- mice (n = 5/group) were exposed to S. rectivirgula (150 μg) three times a week for three or six weeks.
- a BAL was performed and the recovered cells were counted using trypan blue dye exclusion.
- b Cells isolated from the BAL fluid were incubated with antibodies to various cell surface markers and analyzed by flow cytometry to determine the cellular composition of the BALF. CD4+ T cells were identified as βTcR+/CD45+/CD4+; CD8+ T cells - βTcR+/CD45+/CD8+; PMNs - CD45+/CD11b+/Gr1+; NK cells - CD45+/NK1.1+
- *p < 0.05 compared to WT/S. rectivirgula group