Figure 2From: Macrophage pro-inflammatory cytokine secretion is enhanced following interaction with autologous plateletsFlow Cytometric Analysis of Platelet Phagocytosis. hMDMs were incubated with an excess of fluorescently labeled platelets for 45 min in RPMI media alone (white bars) or containing 10% autologous human serum (striped bars). Macrophage fluorescence was measured by flow cytometry and the average percentage of FL1-positive macrophages after removal of adherent platelets are shown for 3 independent experiments for each sample. Data for control samples are repeated in each panel, and compared to treatment with (A) latrunculin (1 ug/ml), (B) thrombin (0.1 U/ml), or (C) fucoidan (250 ug/ml). Statistically significant differences are indicated with their corresponding p-values. The addition of 10% serum did not result in any statistically significant difference (NS).Back to article page