Figure 6

T-cell proliferation and secreted cytokines stimulated by cocktail treated DCs. MLR performed on CD4⁺ T cells and allogeneic DCs. Mature cocktail stimulated DCs were more potent inducers of T cell proliferation in the MLR than immature DCs. A pretreatment of DCs with dexamethasone for 6 h before addition of cocktails, significantly prevent CD4⁺ T cell proliferation to a level similar to immature DCs. The results are representative of three donors. A) CD4⁺ T cells were cultured with allogeneic DCs for 5 days, and mitomycin-C treated in order to inhibit their proliferation. Proliferation of CD4⁺ T cells was determined in the last 18-24 h of culture. Each column represents the mean cpm of four replicates. Vertical bars represent the SD. B) The amount of IFNγ production by T cells was measured in the supernatants after a restimulation with platebound anti-CD3 and soluble anti-CD28 mAbs for 24 h. In the conditioned media was also measured C) TNFα, and D) IL-13 by ELISA. Each column represents the mean of triplicate wells. Vertical bars represent the SD values.