Figure 5

RT-PCR on white cells of LPS-treated WT and KO mice. Graphical representation of minus ΔΔCt values of WT LPS-treated, KO vehicle-treated and KO LPS-treated calculated versus WT vehicle-treated. a) PTPH1- WT and KO mice displayed a trend in LPS-induced increased expression of TNFα in white cells 30 after treatment, that b) became significant 60 mpt in WT mice; at this time point also a genotype-related difference in TNFα expression was recorded between WT and KO mice; c) at 180 minutes TNFα levels were significantly increased by LPS in both WT and KO mice. d) 60 minutes post challenge LPS-induced 187 fold increase in ccl2 gene expression in WT white cells was detected, and no difference in KO mice e) No difference was detected in ccl2 white cells expression of WT and KO mice, 180 minutes after LPS treatment. 2way Anova followed by T-test; *:p < 0.05; **:p < 0.01; ***:p < 0.001.