Figure 5

Apoptosis detection in biopsies of DNCB-induced rashes treated for 24 h with various nanocrystalline silver-derived solutions. Representative fluorescence images obtained via confocal microscopy for immunohistochemical detection of apoptotic cells in pigs with DNCB-induced rashes after 24 h of various treatments are shown. The first column shows staining by FITC for apoptotic cells (green). The second column shows counterstaining by DAPI for nuclei (blue). The third column shows the combination of apoptotic and nuclear staining. Images in Row A are from the surface (epidermis and upper dermis) of a negative control (no rash) treated with distilled water. Images in Rows B and C are of the skin surface and deep dermis, respectively, of a positive control (rash treated with distilled water). Images in Rows D and E are of the surface and the deep dermis, respectively, of a DNCB-induced porcine rash treated with a nanocrystalline silver-derived solution with a starting pH of 4. Images in Rows F and G are of the surface and the deep dermis, respectively, of a DNCB-induced porcine rash treated with a nanocrystalline silver-derived solution with a starting pH of 5.6. Images in Rows H and I are of the surface and the deep dermis, respectively, of a DNCB-induced porcine rash treated with a nanocrystalline silver-derived solution with a starting pH of 7. Images in Rows J and K are of the surface and the deep dermis, respectively, of a DNCB-induced porcine rash treated with a nanocrystalline silver-derived solution with a starting pH of 9. The scale bar in the far right image in Row A represents 20 μm.