Figure 4

Effect of GW0742-treatment on IkB-α degradation and NF-kB p-65 activation. By Western Blot analysis, a basal level of IkB-α was detected in the lung tissue from sham-operated animals, whereas in zymosan-induced mice IkB-α levels were substantially reduced (a, see densitometric analysis a1). Treatment with GW0742 significantly increases the levels of IkB-α, after zymosan injection. Moreover, at 18 h following zymosan-treatment, the levels of NF-kB p-65 subunit protein in the nuclear fractions of the lung tissue were also significant increased compared to the sham-operated mice (b, see densitometric analysis b1). The levels of NF-kB p-65 protein were significantly reduced in the nuclear fractions of the lung tissues from animals that had received GW0742 treatment (b, see densitometric analysis b1). β-actin (a) and Laminin B1 (b) were used as internal control. The result in a1 and b1 are expressed as mean ± S.E. mean from five blots. P < 0.01 vs sham, °P < 0.01 vs zymosan + vehicle.