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Figure 3 | Journal of Inflammation

Figure 3

From: CCR5 signalling, but not DARC or D6 regulatory, chemokine receptors are targeted by herpesvirus U83A chemokine which delays receptor internalisation via diversion to a caveolin-linked pathway

Figure 3

U83A specifically chemoattracts CCR5 bearing primary human leukocytes. PBMCs were plated out on a Neuroprobe chemotaxis apparatus. Lower chambers were filled with a range of chemokines. After 90 minutes incubation, cells remaining on top of the filter were removed and the migrated cells, in the wells below the filter, collected and stained for CCR2 and CCR5. Red peak denotes the background cell migration (buffer only) and green denotes the test peptide/chemokine. CCR2 antibody used was linked to PE and CCR5 antibody linked to FITC. The top, second and third panels show flow cytometry results for cells exposed to 1 nM of CCL2, U83A-N or U83A, respectively. Migration of CCR2 bearing cells were shown towards wells containing CCL2, and CCR5 bearing cells towards those with U83A. Experiments were repeated three times, one representative result shown. For the U83A treatment, the CCR5 specific results from two further donors are shown in the bottom panel. There was no specific chemotaxis detected using the negative control with migration buffer only.

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Journal of Inflammation

ISSN: 1476-9255

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