Figure 6
From: Is inflammation a consequence of extracellular hyperosmolarity?
Pro-inflammatory cytokines transcription following exposure of HT-29 and T24 cells to glycol-derived compounds. Cytokine transcription was analyzed by RNase Protection Assay performed on RNA from human HT-29 (A) or T24 (B) cells exposed for 24 hours to 900 mOsm Mannitol (Man), Propylene Glycol (Prop) or Glycerol (Gly) media as compared to controls (M: normal medium; C: isosmotic control). Hyperosmolarity increases the level of transcription of IL-8, IL-6, IL-1β and TNF-α. GAPDH transcription is not altered.