Figure 2

NF-κB activation in LPS stimulated RAW 264.7 cells. RAW cells were stimulated with LPS (1 μg/ml) for between 0–5 hours. Cells were harvested and nuclear NF-κB-p65 levels were assessed by Western blotting. (a) Representative Western blot of p65 expression. Equal amounts of nuclear proteins are loaded onto each lane. (b) Graphical representation of % increase in p65 nuclear localisation shown in (a) above. Data is presented as mean ± sem, *p < 0.05, n = 6 independent measurements.