Figure 2
From: A novel electron paramagnetic resonance-based assay for prostaglandin H synthase-1 activity
(a) Sample EPR spectra obtained in the absence (control; PGHS + AA) and presence of aspirin (100 μM or 1 mM) after correction for background autoxidation. EPR settings: B0-field, 3356 gauss; sweep width; 50 Gauss, sweep time, 30 sec; modulation amplitude, 1500 mGauss; microwave power, 20 mW. (b) Mean data for development of EPR signal intensity (AU) with time in the absence (control; PGHS + AA) and presence of aspirin (100 μM). In both cases, substrate (AA) was added at t = 0 min. P = 0.02, 2-way ANOVA, repeated measures: n = 9–12.