Figure 4From: Early effects of Staphylococcus aureus biofilm secreted products on inflammatory responses of human epithelial keratinocytesNitrite assay. HEKa cell culture supernatants were collected and treated with Griess Reagent and absorbance was measured at 570nm. Measurements were taken at 4 and 8 hours. Measurements are reported in μM. ANOVA and Tukey’s Honestly Significant Difference were performed to identify statistically significant differences. Results represent the mean and standard deviation of three independent experiments. (#) indicates a statistically significant difference between the PCM and BCM and (*) indicates a statistically significant difference between media and BCM (p < 0.05).Back to article page