Figure 5

Long-time co-incubation of primary osteoblasts with GTE improves osteogenic differentiation and expression of osteogenic genes. Osteoblasts were stimulated for 14 (A) with 1 μg/ml, 0.1 μg/ml and 0.01 μg/ml GTE combined with 50 μM H₂O₂. Mineralization was visualized with alizarin red and afterwards quantified. Also a von Kossa staining was prepared to visualize the mineralization (B) (N = 4, n = 2). After 7 days stimulation with GTE combined with 50 μM H₂O₂, gene expression of osteocalcin (C) and collagen1A1 (D) were measured via qPCR. (N = 3, n = 3). Bars represent mean ± s.e.m, *p < 0.05, **p < 0.01, ***p < 0.001 (ANOVA/Bonferroni).