Figure 6From: Inhibitory effects of heat shock protein 90 blockade on proinflammatory human Th1 and Th17 cell subpopulations17-DMAG disrupts Lck activation. Analysis of Lck in PBMCs stimulated with 1 μg/ml plate-bound anti-CD3 antibody in absence and presence of different concentrations of 17-DMAG for 24 hours. Lck phosphorylation at Tyr394 position was analyzed in lysates of these cell cultures by immunoblotting. Protein concentration was expressed relative to the β-actin level using densitometry measurements. The results are presented as mean values ± SEM of n = 3 healthy blood donors. *P < 0.05.Back to article page