Target | Silencing by treatmenta |
---|
LPS/IFN-γ: | - | + | + |
SiRNA: | IL-12p40 | Mut.siRNA | IL-12p40 |
β-Actin | - | - | - |
IL-12p40 | - | - | + |
IL-12p35 | - | - | - |
IL-23p19 | - | - | - |
IFN-γ | - | - | - |
IFN-β | - | - | - |
TNF-α | - | - | - |
IL-4 | - | - | - |
IL-5 | - | - | - |
IL-6 | - | - | - |
IL-10 | - | - | - |
- aJ774 cells were incubated with or without LPS/IFN-γ and specific or control siRNA as described in the materials and methods section. mRNA for different targets were detected by sqRT-PCR. In this table silencing (+) is defined as the loss of a visible band from stimulated cultures; the - symbol indicates either no loss of a visible band from stimulated (siRNA + LPS/IFN-γ) cultures, or no visible alteration (induction or loss) in unstimulated (siRNA – LPS/IFN-γ) cultures. All results represent at least two experiments performed in triplicate.